The main product of our research effort is the presentation of the results in journal articles. On this page you will find descriptions of our latest research. For more extensive publication lists please see our CVs.
Michael T. A. Nguyen, Martin Vincent Gobry, Néstor Sampedro Vallina, Georgios Pothoulakis, Ebbe Sloth Andersen
This study developed a method that assembles small synthetic genes containing repetitive sequences by splitting them into short synthons and using Golden Gate Assembly, achieving high-fidelity construction of challenging repetitive genes.
ACS (04/03/2024)
Ewan K. S. McRae, Christopher J. K. Wan, Emil L. Kristoffersen, Kalinka Hansen, Edoardo Gianni, Isaac Gallego, Joseph F. Curran, James Attwater, Philipp Holliger, and Ebbe S. Andersen
This study used cryo-EM and mutation analysis to reveal the 3D structure and key interactions of a triplet polymerase ribozyme heterodimer, explaining how its subunits and kissing-loop contacts enable efficient templated RNA synthesis.
PNAS (11/01/2024)
Sampedro Vallina, McRae, Geary, Andersen*
The RNA origami method was used to construct robotic devices that mechanically trap and release RNA functional motifs. Model and map: (to be released soon)
Science Advances (22/05/2023) - bioRxiv 2023
Nguyen*, Gobry, Sampedro Vallina, Pothoulakis, Andersen*
We have developed a method to assemble synthetic genes with repetitive elements, which can be used to construct RNA origamis with multiple aptamers on the same structure. We were forced to develop such a technique because many gene synthesis companies does not allow synthesis of repetitive elements.
ACS Synthetic Biology (accepted Feb 5) - Preprint (Nov 23, 2022).
McRae, Wan, Kristoffersen, Hansen, Gianni, Gallego, Curran, Attwater, Holliger*, Andersen*
In collaboration with the Holliger lab we here describe the first structure of an RNA polymerase ribozyme and determine its fitness landscape. We obtain the 5-Å structure of the apoenzyme and suggest a model for the holoenzyme. Model and map: 8T2P, EMD-40984
PNAS (Jan 11, 2024) - Preprint (Aug 3, 2023) - Preprint (Aug 29, 2022) - Preprint (Aug 23, 2022).
McRae, Rasmussen, Liu, Bøggild, Nguyen, Sampedro Vallina, Boesen, Pedersen, Ren, Geary, Andersen*
We reveal the solution structure of RNA origami by cryo-EM and find important details about kissing loops and crossovers. In addition, we discover an interesting folding trap and demonstrate a nanosatellite shape. Read our press release. Structural data: 7PTQ - 7PTS - 7QDU - 7PTK - 7PTL.
Nature Nanotechnology (Feb 27, 2023) - Preprint (Sep 1, 2022).
Roles of dimeric intermediates in RNA-catalyzed rolling circle synthesis
Kristoffersen, McRae, Sørensen, Holliger*, Andersen*
bioRxiv 2024
An RNA origami robot that traps and releases a fluorescent aptamer
Sampedro Vallina, McRae, Geary, Andersen*
Science Advances (accepted Feb 6 2024) - bioRxiv 2023
Enzymatic assembly of small synthetic genes with repetitive elements
Nguyen*, Gobry, Sampedro Vallina, Pothoulakis, Andersen*
ACS Synthetic Biology (accepted Feb 5 2024) - bioRxiv 2022
Cryo-EM structure and functional landscape of an RNA polymerase ribozyme
McRae, Wan, Kristoffersen, Hansen, Gianni, Gallego, Curran, Attwater, Holliger*, Andersen*
PNAS (Jan 11, 2024) - Preprint (Aug 3, 2023)
RNA target highlights in CASP15: Evaluation of predicted models by structure providers
Kretsch, Andersen, Bujnicki, Chiu, Das, Luo, Masquida, McRae, Schroeder, Su, Wedekind, Xu, Zhang, Zheludev, Moult, Kryshtafovych
PROTEINS: Structure, Function and Bioinformatics 2023
Preprint: AUthorea
RNA origami scaffolds facilitate cryo-EM characterization of a Broccoli–Pepper aptamer FRET pair
Sampedro Vallina, McRae, Hansen, Boussebayle, Andersen*
Nucleic Acid Research 2023
